Good morning people. I know you must be asking what is this person doing up so late writing a blog? Is she in love with this blog or has she some what become attached. Well I beg to differ, I do this because the learning of biochemistry is fun and the learning and discovery of biochemistry must go on!!!! (LOL come on don’t look at me like I am lying)
So we were doing proteins and I learned this new concept called the Anfinsen Experiment. This experiment was used to prove that the information which is required for the 3d structure of a protein is contained within the primary structure ( the amino acid sequence of the protein).
This was determined by an experiment. I would aim to describe this experiment to you:
The protein which was used was an enzyme ribonuclease. GnHCL and mercaptoethanol was added to the protein. This was to denature the protein. the GnHCL is an example of a chaotrope and disrupted the hydrophobic interactions within the tertiary structure. A chemical which could have replaced the GnHCL is urea. Even though the hydrophobic interactions are the main reason for the 3d structure other bonds are prensent within the 3d structure. The strongest bonds which are present are di-sulfide bonds.
Di-sulfide bonds result from the oxidation of two cysteine molecules in order to form one cystine molecules. The mercaptoethanol is added to reduce the disulphide bonds which was cause the protein to unfold completely.
Now protein folding will not be present as the bonds which enabled these folding have been reduced. The solution was then placed in a dialysis tubing. This tubing would contain many small pores which would allow the smaller molecules to pass through. These small molecules would be GnHCL and mercaptothanol. However the unfolded proteins would remain inside the tubing as it is too large to pass through. Once the solutions which promote the breaking of bonds are removed the protein refolds into the 3d structure.
From this I was wondering if possibly the body could contain a mechanism which could remove the substances which cause the denaturation of enzymes? I know its a long fetched thought as the body is not perfect, but I am off to research that one.